Isolation and sequence analysis of the gene encoding triose phosphate isomerase from Zygosaccharomyces bailii

The ZbTPI1 gene encoding triose phosphate isomerase (TIM) was cloned from a Zygosaccharomyces bailii genomic library by complementation of the Sacchar omyces cerevisiae tpi1 mutant strain. The nucleotide sequence of a 1.5 kb f ragment showed an open reading frame (ORF) of 746 bp, encoding a protein of 248 amino acid residues. The deduced amino acid sequence shares a high deg ree of homology with TIMs from other yeast species, including some highly c onserved regions. The analysis of the promoter sequence of the ZbTPI1 revea led the presence of putative motifs known to have regulatory functions in S . cerevisiae. The GenBank Accession No. of ZbTPI1 is AF325852. Copyright (C ) 2001 John Wiley & Sons, Ltd.