Purpose: To evaluate the microhardness of enamel treated with two different
10% carbamide peroxide bleaching materials at different time intervals. Ma
terials and Methods: Two bleaching agents were analyzed: Opalescence (OPA)
and Rembrandt (REM). The control group (CON) consisted of dental fragments
maintained in artificial saliva. Bleaching was accomplished for 8 hrs per d
ay and stored during the remaining time in an individual recipient with art
ificial saliva. Enamel microhardness testing was performed before the initi
al exposure to the treatments and after 1, 7, 14, 21, 28, 35 and 42 days. R
esults: The ANOVA, followed by the Bartlet and Tukey tests, showed signific
ant differences for treatments (P < 0.00001) from day 7-day 42. From the 7t
h to the 14th day, OPA presented an increase of enamel microhardness over t
ime while REM presented a decrease of microhardness. Statistical difference
s were not found between REM and the control group (OPA > CON = REM). From
the 21st-35th day, enamel fragments bleached with OPA and REM presented a d
ecrease of microhardness. Statistical differences of microhardness were ver
ified among all the treatments (OPA > CON > REM). On the day 42, statistica
l differences were not found between OPA and the control group, but they we
re found between REM and the control group (OPA = CON > REM). The polynomia
l regression showed an increase of microhardness for OPA until the 21st day
, followed by a decrease of microhardness up to the 42nd day. A decrease of
microhardness for REM was verified. There were alterations in enamel micro
hardness as a function of bleaching time when using the two different 10% c
arbamide peroxide whiteners. Over a 42-day treatment time, bleaching with R
EM agent caused a decrease in enamel microhardness. The OPA agent initially
increased the microhardness, then returned to the control level. Different
bleaching materials with the same concentration of carbamide peroxide have
different effects on the enamel.