Distribution of some rare earth elements and their binding species with proteins in human liver studied by instrumental neutron activation analysis combined with biochemical techniques

Citation
Cy. Chen et al., Distribution of some rare earth elements and their binding species with proteins in human liver studied by instrumental neutron activation analysis combined with biochemical techniques, ANALYT CHIM, 439(1), 2001, pp. 19-27
Citations number
21
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICA CHIMICA ACTA
ISSN journal
00032670 → ACNP
Volume
439
Issue
1
Year of publication
2001
Pages
19 - 27
Database
ISI
SICI code
0003-2670(20010717)439:1<19:DOSREE>2.0.ZU;2-Y
Abstract
Distribution patterns of three rare earth elements and their binding specie s with proteins in the human liver samples were studied by instrumental neu tron activation analysis combined with biochemical techniques. It was indic ated that La, Ce and Sm were highly concentrated in microsomes, although th e total content of this fraction was the least among all of the liver organ elles, which was similar with the behavior of Ca and Fe. More than 85% of L a, Ce and Sm was present in the cytosolic protein fractions separated by am monium sulfate and ethanol. It indicated that these elements were mainly in corporated into protein. It was also revealed by means of size-exclusion ch romatography that at least three La-binding proteins (M-x: 335 +/- 50, 94.5 +/- 15.4, 13.6 +/- 3.8 kD), three Ce-binding proteins (M-w: 335 +/- 50, 85 .1 +/- 12.0, 22.8 +/- 6.3 kD) and about four Sm-binding proteins (M-w: 335 +/- 70, 82.1 +/- 5.4, 32.3 +/- 5.8 and 13.6 +/- 4.5kD) were present in the supernatant fraction of human liver. Most of La, Ce and Sm were found in th e high-molecular weight protein region. (C) 2001 Elsevier Science B.V. All rights reserved.