Chemiluminescence detection of paracetamol by a luminol-permanganate basedreaction

The flow injection analysis of paracetamol was developed based on its inhib itory effect on a luminol-permanganate chemiluminescence system. With a sys tem containing solutions of 2.5 mM luminol, 5.0 muM KMnO4 and pH 12.0 phosp hate buffer (K2HPO4/NaOH), a detection limit of 1.0 x 10(-8) M (1.5 ppb) an d a linear dynamic range of 2.5 x 10(-8) to 2.5 x 10(-7) M can be obtained. A repetitive injection of 1.0 x 10-7 M paracetamol solutions gave satisfac tory reproducibility with a relative standard deviation of 2.3% (n = 12). P ossible interferences from major excipients in pharmaceuticals and other re lated compounds were also studied. Contents of paracetamol in two locally a vailable pharmaceuticals were analyzed. From the recovery of 98.2-104.4% ob tained, the feasibility of the developed method for real sample analysis wa s confirmed. (C) 2001 Elsevier Science B.V. All rights reserved.