Isolation of 105 microsatellite loci from an ovine genomic library enriched for microsatellites

This paper reports on the development of a small-insert (similar to 700 bp) total-genomic library for sheep specifically designed for enrichment for m icrosatellite (ms) loci. Four enriched libraries were prepared by amplifica tion of the primary library with CA(15), CA(11), TG(15) and TG(11) oligonuc leotide primers. A total of 11,020 clones was recovered, screened for dinuc leotide repeats and over 500 positive clones sequenced. Sequence analysis i ndicated low clone redundancy and yielded 105 new ovine ms loci. Seventy-tw o percent of the new loci were found to be polymorphic in the sires of the AgResearch International Mapping Flock (IMF). The 105 new microsatellite lo ci increase the number of microsatellites available for sheep by >7%.