Antibacterial effect of antibiotic solution on cellular viability in canine veins

Pretreatment of tissue by using antibiotics is a critical step to prevent m icrobial contamination before venous transplantation. In this study, the op timal time and temperature of antibiotic solution treatment for maintaining cellular viability with antibacterial effect were investigated. The antibi otic-nutrient solutions were composed of cefoxitin, lincomycin, vancomycin, and polymyxin B in RPMI-1640 medium. After various antibiotic solution tre atment times (4, 8, and 12 h) and temperatures (4, 25, and 37 degreesC), th e viabilities of cells dissociated from veins (jugular vein, femoral vein, superior vena cava, and inferior vena cava) were determined. Double stainin g by Griffonia simplicifolia agglutins-fluorescein isothiocyanate (GS1-FITC ) and propidium iodide was used. To measure the antibacterial effect of the antibiotic solution, canine veins were artificially infected by 3 kinds of bacteria (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoni ae) and were treated by antibiotic solutions as viability test conditions. After the treatment with the antibiotic solution, the tissue was minced, an d the homogenized tissue fraction was cultured on standard method agar. The colony that seemed to be resistant to the antibiotic solution was counted. At 37 and 25 degreesC, the viability of whole cells decreased significantl y Asymptotic Significance 2-tailed (Asymp.Sig 2-tailed) < 0.05 after 4 h of antibiotic solution treatment, whereas at 4 degreesC it began to reduce si gnificantly after 8 h of treatment. By antibiotic solution treatment at all 3 temperatures for 4 h, no significant difference in viability of the endo thelial cells and whole cells was observed. To maintain the donor vein's ce llular viability until transplantation, antibiotic solution treatment for 4 h at 4 degreesC is assumed to be appropriate.