Improvements in the determination of manganese peroxidase activity

Authors
Grgic, I Podgornik, H Berovic, M Perdih, A
Citation
I. Grgic et al., Improvements in the determination of manganese peroxidase activity, BIOTECH LET, 23(13), 2001, pp. 1039-1042
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
13
Year of publication
2001
Pages
1039 - 1042
Database
ISI
SICI code
0141-5492(2001)23:13<1039:IITDOM>2.0.ZU;2-Q
Abstract
The existing method of determining the activity of manganese peroxidase (Mn P), produced by Phanerochaete chrysosporium, was improved. 2,6-Dimethoxyphe nol at 80 mM was used as a substrate and, after the decolorization of the r eaction mixture, H2O2 was added and the initial reaction rate was used to d etermine MnP activity.