Improved production of recombinant rotavirus VP6 in sodium butyrate-supplemented suspension cultures of transgenic tomato (Lycopersicon esculentum Mill.) cells

Authors
Kim, CH Kim, KI Hong, SH Lee, YH Chung, IS
Citation
Ch. Kim et al., Improved production of recombinant rotavirus VP6 in sodium butyrate-supplemented suspension cultures of transgenic tomato (Lycopersicon esculentum Mill.) cells, BIOTECH LET, 23(13), 2001, pp. 1061-1066
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
13
Year of publication
2001
Pages
1061 - 1066
Database
ISI
SICI code
0141-5492(2001)23:13<1061:IPORRV>2.0.ZU;2-B
Abstract
Recombinant rotavirus VP6 production was 0.49 mg l(-1) when 10 mM sodium bu tyrate was added to the suspension culture of transgenic tomato cells after 6 days' incubation. Recombinant VP6 production increased to 0.73 mg l(-1), when the culture was supplemented with 10 mM sodium butyrate on day 6 and 9 after incubation. Total protein extracts of transgenic tomato cells from sodium butyrate-supplemented culture were examined by two-dimensional elect rophoresis: 14 proteins were up-regulated and 7 down-regulated.