FIRST STEPS OF THE INTERACTION OF THE TEMPERATE PHAGE EMPTY-SET-O4 WITH PSEUDOMONAS-AERUGINOSA HOST-CELLS

The first steps of the interaction of the temperate pilus-dependent ph age OO4 with susceptible and empty set 04-lysogenic cells of Pseudomon as aeruginosa were investigated. The empty set 04-lysogenic cells reta ined their ability to adsorb viral particles on the pili. However, aft er the translocation to the cell surface, the bacteriophage failed to infect the immune microorganism. It is assumed that the surface of emp ty set 04-immune cells was modified due to lysogenic conversion, and t his modification prevented the terminal tail fibers of viral particles from interacting with the outer membrane of P. aeruginosa cells.