Ak. Srivastava et al., Induced resistance and control of charcoal rot in Cicer arietinum (chickpea) by Pseudomonas fluorescens, CAN J BOTAN, 79(7), 2001, pp. 787-795
Citations number
59
Categorie Soggetti
Plant Sciences
Journal title
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
Pseudomonas fluorescens isolate 4-92 induced systemic resistance against ch
arcoal rot disease in chickpea (Cicer arietinum L.) caused by Macrophomina
phaseolina (Tassi) Goidanich. Time-course accumulation of pathogenesis-rela
ted (PR) proteins (chitinases and glucanases) in chickpea plants inoculated
with P. fluorescens was significantly (P = 0.05) higher than in control pl
ants. The level of chitinases and glucanases increased by 6.6- to 7-fold up
to 4 days postinoculation; thereafter, little decrease in the activity of
PR proteins was observed. Root-colonizing populations of P. fluorescens wer
e at a maximum 2 days after transplantation at different inoculum concentra
tions, and decreased over time. Inoculation of root tips of chickpea by P.
fluorescens, 2,6-dichloroisonicotinic acid, and o-acetylsalicylic acid indu
ced systemic resistance against charcoal rot. Disease was 33 to 55.5% highe
r in control plants than in plants inoculated with chemical inducers or P.
fluorescens. Single treatment of plants with P. fluorescens increased disea
se resistance by 33%, whereas combined application of P. fluorescens with e
ither of the chemical inducers was most effective in inducing the resistanc
e by 2- to 2.25-fold. The time-course study shows that an interval of at le
ast 2 days was required between induction treatment and challenge inoculati
on. Biocontrol efficacy of P. fluorescens against charcoal rot disease in c
hickpea was demonstrated under greenhouse conditions.