Determination of dissociation constants of complicated compounds by capillary zone electrophoresis

In this work, the whole theoretical methods for the determination of PKa1 a nd pK(a2) of complicated compounds are proposed by capillary zone electroph oresis. The pK(a) values are achieved by non-linear regression analysis by taking into consideration the effect of activity coefficient. This is the f irst report on determining the dissociation constants of gastrodin, magnolo l, honokiol, quercetin, curcumin, diethylstilbestrol, 4-acetamidophenol, eu genol and paeonol.