Objective: To determine within the setting of isocaloric, isonitrogenous en
teral diets whether a diet that supplies most of its calories from fat or c
arbohydrate would he most beneficial at limiting muscle protein wasting in
catabolic illness.
Design: Prospective, randomized, crossover trial.
Setting: Academic pediatric burn unit in tertiary medical center.
Patients: Fourteen severely burned (> 40% total body surface area) children
underwent systemic metabolic and cross-leg muscle protein kinetic studies.
Interventions: All were treated clinically in a similar manner, including e
arly excision and grafting, antimicrobial therapy, and isocaloric, isonitro
genous enteral nutritional support. Subjects randomly received either a hig
h-carbohydrate enteral diet (3% fat, 82% carbohydrate, 15% protein), or a h
igh-fat enteral diet (44% fat, 42% carbohydrates, 14% protein) for 1 week a
nd then crossed over to the other diet for a second week.
Measurements and Main Results: On day 5 of each diet, muscle protein kineti
cs were determined from femoral arterial and venous blood samples during a
primed-constant d5-phenylalanine infusion. Indirect calorimetry was used to
determine systemic resting energy expenditure and respiratory quotient. Th
e seven boys and seven girls were 7.1 +/- 1.1 (mean +/- SEM) years old and
suffered burns over 65 +/- 4% of their bodies, with 52 +/- 6% being third-d
egree burns. Muscle protein degradation markedly decreased (p < .01) with a
dministration of the high-carbohydrate diet. Protein synthesis was unaltere
d. Endogenous insulin concentrations increased during the high-carbohydrate
feeding period. No differences in energy expenditure were seen between stu
dy diets,
Conclusions: In severally burned pediatric patients, enteral nutrition supp
lied predominantly as carbohydrate rather than fat improves the net balance
of skeletal muscle protein across the leg. This is attributable to decreas
ed protein breakdown, suggesting a protein-sparing effect of high-carbohydr
ate feedings.