Taxonomy of the unicellular green algal genus Vitreochlamys (Volvocales), based on comparative morphology of cultured material

The taxonomy of Vitreochlamys species (Volvocales, Chlorophyta) was studied with light and electron microscopy; DNA sequence data were obtained for ni ne strains from various localities in Japan, the Czech Republic, Slovak Rep ublic and Germany. The strains could be delineated clearly into six species - V. fluviatilis (Stein) Batko, V. gloeocystiformis (Dill) Nakazawa, comb. nov., V. ordlinata (Skuja) Nakazawa, comb, nov., V. nekrassovii (Korshikov ) Nakazawa, comb. nov., V. aulata (Pascher) Batko, and V. pinguis Nakazawa, sp. nov. - on the basis of differences in cell and protoplast shape, numbe rs of contractile vacuoles and pyrenoids, form and position of the stigma, location of the nucleus, degree of longitudinal striations on the chloropla st surface, and ultrastructure of the pyrenoid and stigma. Although V. ping uis and V. aulata share common chloroplast morphology, including stigma and pyrenoid ultrastructure, these two species can be distinguished clearly by cell shape, the number of contractile vacuoles and location of the nucleus . In addition, phylogenetic sequence data of the rbcL (large subunit of Rub isco, ribulose-1,5-bisphosphate carboxylase/oxygenase) gene from all of the Vitreochlamys strains strongly suggest that V. pinguis from Japan is robus tly separated from the clade composed of four strains of V. aulata from the Czech Republic, Slovak Republic and Japan. The sequence data also showed t hat Vitreochlamys is composed of three clades, which are largely consistent with the ultrastructural features of the stigma and pyrenoids.