Evidence is accumulating that in cell membranes microdomains exist, also re
ferred to as rafts or detergent resistant membranes, In this study, atomic
force microscopy is used to study supported lipid bilayers, consisting of a
fluid phosphatidylcholine, sphingomyelin and cholesterol, Domains were vis
ualized of which the morphology and size depended on the cholesterol concen
tration, The presence of cholesterol was found to induce bilayer coupling.
At 30 mol% cholesterol, a change in percolation phase was observed, and at
50 mol%, when both fluid lipids and solid lipids are saturated with cholest
erol, phase separation was still observed. In addition, we were able to dir
ectly visualize the resistance of domains against non-ionic detergent. (C)
2001 Federation of European Biochemical Societies. Published by Elsevier Sc
ience B.V. All rights reserved.