AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene

Citation
Wcs. Tai et Dk. Banfield, AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene, FEBS LETTER, 500(3), 2001, pp. 177-182
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
500
Issue
3
Year of publication
2001
Pages
177 - 182
Database
ISI
SICI code
0014-5793(20010706)500:3<177:AAATBS>2.0.ZU;2-N
Abstract
SNAREs are membrane-associated proteins that play a central role in vesicle targeting and intra-cellular membrane fusion reactions in eukaryotic cells , Here we describe the identification of AtBS14a and AtBS14b, putative SNAR Es from Arabidopsis. thaliana that share 60% amino acid sequence identity, Both AtBS14a and BS14b are dosage suppressors of the temperature-sensitive growth defect in sft1-1 cells and overexpression of either AtBS14a or AtBS1 4b can support the growth of sft1 Delta cells but not bet1 Delta cells. The se data together with structure-function and biochemical studies presented herein suggest that AtBS14a and AtBS14b share properties that are consisten t with them being members of the Bet1/Sft1 SNARE protein family. (C) 2001 P ublished by Elsevier Science B.V. on behalf of the Federation of European B iochemical Societies.