Wcs. Tai et Dk. Banfield, AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene, FEBS LETTER, 500(3), 2001, pp. 177-182
SNAREs are membrane-associated proteins that play a central role in vesicle
targeting and intra-cellular membrane fusion reactions in eukaryotic cells
, Here we describe the identification of AtBS14a and AtBS14b, putative SNAR
Es from Arabidopsis. thaliana that share 60% amino acid sequence identity,
Both AtBS14a and BS14b are dosage suppressors of the temperature-sensitive
growth defect in sft1-1 cells and overexpression of either AtBS14a or AtBS1
4b can support the growth of sft1 Delta cells but not bet1 Delta cells. The
se data together with structure-function and biochemical studies presented
herein suggest that AtBS14a and AtBS14b share properties that are consisten
t with them being members of the Bet1/Sft1 SNARE protein family. (C) 2001 P
ublished by Elsevier Science B.V. on behalf of the Federation of European B
iochemical Societies.