Inactivation of Trichinella spiralis by high pressure treatment

In experimental studies, the inactivation of T. spiralis in pork was invest igated by high hydrostatic pressures. Trichinella contaminated meat was pre ssurised at 50, 100, 150, 200 und 250 MPa (temperature: 20 degreesC; time: 20 and/or 30 Min.). In another study, the efficacy of high pressure treatme nt was examined at 100 and 150 MPa respectively, in dependence of temperatu re (5, 15, 25 degreesC) and time (10, 20, 30 Min.). The inactivation of lar vae was checked in-vivo and in-vitro (vitality test of larvae) as well. Lar vae pressurised at 50 MPa proved to be infective in laboratory animals. Eve n if the pressure was increased to 100 or 150 MPa, a complete inactivation was observed only when the temperature was decreased to 5 degreesC. If the pressure level of the treatment was elevated to 200 MPa, larve were inactiv ated in all series of tests. According to results of in vitro and in vivo e xamination, there is obviously a close relation between both methods. This has to be proved in further studies.