H. Braasch et al., Records of Bursaphelenchus spp. (Nematoda, Parasitaphelenchidae) in coniferous timber imported from the Asian part of Russia, FOREST PATH, 31(3), 2001, pp. 129-140
Coniferous wood imported from the Asian part of Russia was surveyed in Germ
any (Mukran ferry terminal, highway and railway border station in Frankfurt
/Oder) and Austria (railway in Marchegg, Retz and Wr. Neustadt, Lower Austr
ia). The consignments consisted of mixed timber of Pinus/Picea or Pinus/Lar
ix. Out of 625 samples investigated, 51 samples (8.5%) yielded eight Bursap
helenchus species. Bursaphelenchus mucronatus was found 42 timer in Pinus,
Picea and Larix wood, Birsaphelenchus hylobianum and Bursaphelenchus fraudu
lentus were detected twice, in Pinus/Larix and in Larix, respectively. The
following species were each found once: Bursaphelenchus leoni in mixed timb
er of Pinus/Picea, Bursaphelenchus 'borealis' in Pinus, Bursaphelenchus hel
lenicus and Bursaphelenchus paracorneolus in Larix. One sample of Larix woo
d from Krasnovarsk region contained a few specimens corresponding to Bursap
helenchus paracorneolus. Bursaphelenchus; mucronatus was present in about 3
0% of the samples showing signs of insect attack. A Monochamus species was
found in a sample from Irkutsk. Most of the B. mucronatus isolates found be
longed to the European genotype, whereas the East Asian genotype was found
in three instances. This is the first report of B. fraudulentus, B. helleni
cus, B. leoni, B. paracorneolus and the East Asian genotype of B. mucronatu
s in Russia. Bursaphelenchus hylobianum is the only species found in Russia
n wood and nor in Europe so far. The species were identified morphologicall
y and by internal transcribed spacer (ITS)-restriction fragment length poly
morphism (RFLP) technique. Species-specific ITS-RFLP patterns a cre establi
shed for B. hylobianum. In the case of the isolate morphologically correspo
nding to B. xylophilus, DNA extraction from the available low number of spe
cimens failed to yield sufficent rDNA for ITS-RFLP analysis.