Records of Bursaphelenchus spp. (Nematoda, Parasitaphelenchidae) in coniferous timber imported from the Asian part of Russia

Citation
H. Braasch et al., Records of Bursaphelenchus spp. (Nematoda, Parasitaphelenchidae) in coniferous timber imported from the Asian part of Russia, FOREST PATH, 31(3), 2001, pp. 129-140
Citations number
28
Categorie Soggetti
Plant Sciences
Journal title
FOREST PATHOLOGY
ISSN journal
14374781 → ACNP
Volume
31
Issue
3
Year of publication
2001
Pages
129 - 140
Database
ISI
SICI code
1437-4781(200106)31:3<129:ROBS(P>2.0.ZU;2-Y
Abstract
Coniferous wood imported from the Asian part of Russia was surveyed in Germ any (Mukran ferry terminal, highway and railway border station in Frankfurt /Oder) and Austria (railway in Marchegg, Retz and Wr. Neustadt, Lower Austr ia). The consignments consisted of mixed timber of Pinus/Picea or Pinus/Lar ix. Out of 625 samples investigated, 51 samples (8.5%) yielded eight Bursap helenchus species. Bursaphelenchus mucronatus was found 42 timer in Pinus, Picea and Larix wood, Birsaphelenchus hylobianum and Bursaphelenchus fraudu lentus were detected twice, in Pinus/Larix and in Larix, respectively. The following species were each found once: Bursaphelenchus leoni in mixed timb er of Pinus/Picea, Bursaphelenchus 'borealis' in Pinus, Bursaphelenchus hel lenicus and Bursaphelenchus paracorneolus in Larix. One sample of Larix woo d from Krasnovarsk region contained a few specimens corresponding to Bursap helenchus paracorneolus. Bursaphelenchus; mucronatus was present in about 3 0% of the samples showing signs of insect attack. A Monochamus species was found in a sample from Irkutsk. Most of the B. mucronatus isolates found be longed to the European genotype, whereas the East Asian genotype was found in three instances. This is the first report of B. fraudulentus, B. helleni cus, B. leoni, B. paracorneolus and the East Asian genotype of B. mucronatu s in Russia. Bursaphelenchus hylobianum is the only species found in Russia n wood and nor in Europe so far. The species were identified morphologicall y and by internal transcribed spacer (ITS)-restriction fragment length poly morphism (RFLP) technique. Species-specific ITS-RFLP patterns a cre establi shed for B. hylobianum. In the case of the isolate morphologically correspo nding to B. xylophilus, DNA extraction from the available low number of spe cimens failed to yield sufficent rDNA for ITS-RFLP analysis.