Comparison of selective media for the recovery and enumeration of probiotic yeasts from animal feed

Six selective media (acidified malt extract agar, CHROMagar Candida, dichlo ran rose bengal chloramphenicol, molybdate, oxytetracycline glucose yeast e xtract and Petrifilm Yeast and Mould agar) were examined for the recovery o f three yeasts commonly used in animal feeds as probiotic additives (Candid a pintolopesii, C. saitoana and Saccharomyces cerevisiae). The highest reco very was obtained on oxytetracycline glucose yeast extract agar, although t his was susceptible to overgrowth by moulds. CHROMagar Candida also gave go od recovery and species were easily discriminated by the differential colou r formation of colonies. (C) 2001 Published by Elsevier Science B.V.