Comparison of VIDAS enzyme-linked fluorescent immunoassay using Moore swabsampling and conventional culture method for Salmonella detection in bulk tank milk and in-line milk filters in California dairies

A comparison of the VIDAS Salmonella (SLM) assay using the manufacturer's r ecommended sampling method or a Moore swab sampling method demonstrated tha t the Moore swab method detected a greater number of positive samples (83.0 % vs. 67.92%). When results using a conventional culture technique were com pared to the VIDAS SLM assay using Moore swab sampling, there was good agre ement (97.5%). When the VIDAS SLM assay was used to test in-line milk filte rs and compared to results from the conventional culture method, there was also good correlation between test. results (95.57%). Overall, the VIDAS SL M assay using a modified sampling method compared favorably to the conventi onal culture method and had the advantages of taking less time to obtain a negative or presumptive positive result, being less technically complicated and requiring less screening of non-lactose fermenting colonies from negat ive samples. (C) 2001 Elsevier Science B.V. All rights reserved.