Twelve phenotypically similar marine bacteria have been studied by means of
ribotyping. DNA-DNA hybridization and cultural and physiological character
ization. Phylogenetic analysis has been performed of the 16S and 23S rRNA g
enes of two representative strains. Phylogenetically. they belong to the Vi
brio/Photobacterium branch of the gamma -Proteobacteria and they share all
of the properties that define the genus Vibrio. The strains represent a new
Vibrio species that is phenotypically similar to Vibrio splendidus, Howeve
r, resistance to the vibriostatic agent O129 and production of acid from se
veral carbohydrates allow differentiation between V. splendidus and the pro
posed new species. The DNA G + C content of the proposed type strain is 44.
0 mol%, The name Vibrio lentus sp. nov. is proposed for the new species and
strain 40M4(T) (= CECT 5110(T) = DSM 13757(T)) is the type strain.