Vascular responses to beta-adrenoceptor subtype-selective agonists with and without endothelium in rat common carotid arteries

1 Using the cannula inserting method, vasodilator responses to beta -adreno ceptor agonists (isoprenaline, denopamine and procaterol) were investigated in isolated and perfused rat common carotid arteries. 2 Each beta -adrenoceptor agonist induced a vasodilation in preparations pr econstricted by phenylephrine in a dose-related manner. The potencies were in the order of isoprenaline > procaterol >> denopamine. 3 Denopamine-induced dilations were significantly inhibited by 1 nmol betax olol (a selective beta (1)-adrenoceptor antagonist), but it was not influen ced by 1 nmol ICI 118,551 (a selective beta (2)-adrenoceptor antagonist). O n the other hand, procaterol-induced vasodilations were significantly inhib ited by 1 nmol ICI 118,551 but not modified by 10 nmol betaxolol. 4 ACh-induced vasodilations disappeared after intraluminal saponin injectio n to remove endothelium, but procaterol- and denopamine-induced dilations w ere not modified by removal of the endothelium. 5 Pretreatment with L-N-G-nitroarginine methyl ester (L-NAME) readily inhib ited ACh-induced vasodilations. However, neither procaterol- or denopamine- induced vasodilation was modified by L-NAME treatment. 6 From these results, it is concluded that in the rat common carotid arteri es (1) there are abundant beta (2)- and a few beta (1)-adrenoceptors, and ( 2) there is no participation of the endothelium-dependent mechanism in beta -adrenoceptor mediated vasodilations.