Sticky DNA, a self-associated complex formed at long GAA center dot TTC repeats in intron 1 of the frataxin gene, inhibits transcriptions

Citation
N. Sakamoto et al., Sticky DNA, a self-associated complex formed at long GAA center dot TTC repeats in intron 1 of the frataxin gene, inhibits transcriptions, J BIOL CHEM, 276(29), 2001, pp. 27171-27177
Citations number
48
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
276
Issue
29
Year of publication
2001
Pages
27171 - 27177
Database
ISI
SICI code
0021-9258(20010720)276:29<27171:SDASCF>2.0.ZU;2-1
Abstract
Friedreich's ataxia (FRDA) is an autosomal recessive neurodegenerative dise ase caused by the expansion of GAA.TTC repeats in the first intron of the f rataxin (X25) gene. FRDA patients carrying two expanded GAA.TTC repeats sho w very low levels of mature frataxin mRNA and protein. A novel type of unus ual DNA structure, sticky DNA, was previously found in the expanded GAA.TTC repeats from FRDA patients, To evaluate the effect of sticky DNA on transc ription, in vitro transcription studies of (GAA.TTC), repeats (where n = 9- 150) were carried out using T7 or SP6 RNA polymerase, When a gel-isolated s ticky DNA template was transcribed, the amount of full-length RNA synthesiz ed was significantly reduced compared with the transcription of the linear template, Surprisingly, transcriptional inhibition was observed not only fo r the sticky DNA template but also another DNA molecule used as an internal control in an orientation-independent manner. The molecular mechanism of t ranscriptional inhibition by sticky DNA was a sequestration of the RNA poly merases by direct binding to the complex DNA structure. Moreover, plasmids containing the (GAAGGA.TCCTTC)(65) repeat, which does not form sticky DNA, did not inhibit in vitro transcription, as expected. These results suggest that the role of sticky DNA in FRDA may be the sequestration of transcripti on factors.