Non-aqueous capillary electrophoretic separation of enantiomeric amines with (-)-2,3 : 4,6-di-O-isopropylidene-2-keto-L-gulonic acid as chiral counter ion

(-)-2,3:4,6-Di-O-isopropylidene-2-keto-L-gulonic acid [(-)-DIKGA] has been introduced as a chiral counter ion in non-aqueous capillary electrophoresis . High enantioresolutions (R(s)greater than or equal to3) were obtained for amines, e.g., pronethalol, labetalol and bambuterol. Methanol containing N aOH and (-)-DIKGA was used as the background electrolyte. The counter ion c oncentration and the nature of the injection medium were found to affect th e chiral separation. Covalent coating of the fused-silica capillary reduced the electro-osmotic flow resulting in improved enantioresolutions. (C) 200 1 Elsevier Science B.V, All rights reserved.