Molecular cloning of the self-incompatibility genes S1 and S3 from almond (Prunus dulcis cv. Ferragnes)

Citation
Rc. Ma et Mm. Oliveira, Molecular cloning of the self-incompatibility genes S1 and S3 from almond (Prunus dulcis cv. Ferragnes), SEX PLANT R, 14(3), 2001, pp. 163-167
Citations number
31
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
SEXUAL PLANT REPRODUCTION
ISSN journal
09340882 → ACNP
Volume
14
Issue
3
Year of publication
2001
Pages
163 - 167
Database
ISI
SICI code
0934-0882(200111)14:3<163:MCOTSG>2.0.ZU;2-#
Abstract
Almond (Prunes dulcis) displays gametophytic self-incompatibility. In the w ork reported here, we cloned two novel S-RNase genes from almond cultivar F erragnes (genotype S1S3) using PCR. The S1-RNase gene has the same coding r egion as the Sb gene cloned from almond cultivated in the USA; however, the ir introns are different in sequence. S1 was cloned and sequenced from six different cultivars originating in Europe. The full-length of the S3-RNase gene was cloned using two primers corresponding to the start and stop codon s contexts. Two introns are present in the S3 gene, unique among the S-RNas e genes. Sequence-specific PCR was performed to confirm that the two cloned genes co-segregate with the S-locus using progenies of a controlled cross between Tuono (S1Sf) and Ferragnes (S1S3). Based on the structural differen ces of S-and S-like RNase genes, we discuss the evolutionary relationship b etween the two groups of RNase genes.