The pattern of genetic variation within and among natural populations of br
oomrape (Orobanche crenata Forsk.) from southern Spain was analysed by RAPD
markers. Hierarchical analysis of phenotypic diversity using AMOVA was per
formed to analyse the partitioning of the variation among populations and a
mong individuals. Although most of the genetic diversity was attributable t
o differences among individuals within a population (94.29%), significant p
hi (st) values among populations suggested the existence of phenotypic diff
erentiation. Moreover, corresponding HOMOVA analysis revealed that molecula
r variances were significantly heterogeneous among populations although no
clear grouping pattern could be established. These results are to be expect
ed considering the predominant outcrossing behaviour of O. crenata.