Sequences of the cDNAs and genomic DNAs encoding the S1, S7, S8, and Sf alleles from almond, Prunus dulcis

Partial genomic and cDNA sequences of the RNase alleles S1, S7, S8 and Sf w ere obtained from Prunus dulcis cvs 'Ne Plus Ultra', 'Ferragnes' and 'Non-p areil' 15-1, and IRTA Selection 12-2. Total DNA was extracted from leaves, and cDNA was prepared from total RNA extracted from styles. The partial cDN A sequences of the S1 allele from 'Ferragnes', and the S7 and S8 alleles fr om 'Nonpareil' 15-1, matched those reported in the literature for the allel es Sb, Sc and Sd respectively. The sequences of the S1, S7, S8 and Sf allel es found in genomic DNA contained introns of 562, 1,530, 2,208 and 689 bp r espectively. The exon/intron splice junction sites of all alleles followed the GT/AG consensus sequence rule, and the sequences were found to be highl y conserved.