Two experimental techniques, the DNA-topology assay and the Ames assay, wer
e proved to be suitable for monitoring compounds with a genotoxic potential
and/or with an antimutagenic effect. Both procedures were used in assaying
the acid-mine water (AMW) containing toxic metals and sulfoethyl chitin-gl
ucan (SE-Ch-G), a derivative of chitin-glucan, in which bioprotective activ
ities were detected earlier. It was shown that after toxic metal concentrat
ions were decreased due to AMW dilution to the limits that correspond with
those set by the Slovak Technical Norm (STN) for drinking water, AMW was no
t genotoxic in the Ames assay. As it is possible to detect any single-stran
d DNA (ssDNA) break in the DNA-topology assay, the SE-Ch-G protective effec
t against the ssDNA breaks induced by Fe2+ in the DNA-topology assay was re
corded. SE-Ch-G exhibited the antimutagenic potential after its application
simultaneously with diagnostic mutagens in the Ames assay. These results d
emonstrate the complementarity of both experimental systems. (C) 2001 Elsev
ier Science Ltd. All rights reserved.