In order to elucidate the effects of argon laser irradiation on the la
teral semicircular canal of the guinea pig, the vestibular labyrinth w
as histologically studied after irradiation, using the conventional ce
lloidin method. Irrigation of the external meatus with ice water was u
sed to evaluate the function of the semicircular canal by recording ca
loric nystagmus. When irradiation was performed, a laser probe was app
roximated to the lateral canal, 0.5 to 1 mm away from the surface of t
he canal. Each time, power applied was 1.0 W on the dial of the laser
machine. The duration of irradiation was 0.5 s. The lateral canal was
irradiated one to 15 times. Twenty-five to 87 days after irradiation,
the temporal bones were fixed in Heidenhein-Susa solution, removed, an
d subjected to celloidin processing. The irradiated bony wall of the l
ateral canal demonstrated charring. Lucent areas were observed around
and under the charred area. The semicircular duct showed shrinkage wit
h disappearance of the trabecular mesh. New bone formation was observe
d along the endosteum of the irradiated area. The lateral canal was co
mpletely occluded by ossification with or without fibrosis when suffic
ient energy was applied. The anterior and posterior canals were normal
. Caloric tests using 5 mL of ice water for 5 s failed to elicit nysta
gmus on the irradiated side.