FACTORIAL EXPERIMENTAL-DESIGN AND PRINCIPAL COMPONENT ANALYSIS OF THEINTERACTION OF ANIMAL GLUES WITH POLYMERIC AND SILICA-BASED STATIONARY PHASES IN SIZE-EXCLUSION CHROMATOGRAPHY
Cj. Weatherell et Epc. Lai, FACTORIAL EXPERIMENTAL-DESIGN AND PRINCIPAL COMPONENT ANALYSIS OF THEINTERACTION OF ANIMAL GLUES WITH POLYMERIC AND SILICA-BASED STATIONARY PHASES IN SIZE-EXCLUSION CHROMATOGRAPHY, Journal of chromatography, 669(1-2), 1994, pp. 31-44
The interactions of collagen, Croda glue and VM gelatin which are orga
nic additives employed in the base metals industry, with a polymeric a
nd a derivatized silica stationary phase in size exclusion chromatogra
phy are examined by a 2(3) factorial experimental design. Main factor
coefficients indicate the presence of ion-exclusion effects for all pr
otein solutes on both stationary phases. The propensity toward ion exc
lusion is greater on the silica-based stationary phase. Hydrophobic ad
sorption of collagen and Croda glue was evident on the polymeric phase
. Collagen and Croda glue display identical behaviour on both stationa
ry phases as suggested by their principal components. Anomalous behavi
our of VM gelatin is suggested by both main factor coefficients and pr
incipal component analysis.