FACTORIAL EXPERIMENTAL-DESIGN AND PRINCIPAL COMPONENT ANALYSIS OF THEINTERACTION OF ANIMAL GLUES WITH POLYMERIC AND SILICA-BASED STATIONARY PHASES IN SIZE-EXCLUSION CHROMATOGRAPHY

The interactions of collagen, Croda glue and VM gelatin which are orga nic additives employed in the base metals industry, with a polymeric a nd a derivatized silica stationary phase in size exclusion chromatogra phy are examined by a 2(3) factorial experimental design. Main factor coefficients indicate the presence of ion-exclusion effects for all pr otein solutes on both stationary phases. The propensity toward ion exc lusion is greater on the silica-based stationary phase. Hydrophobic ad sorption of collagen and Croda glue was evident on the polymeric phase . Collagen and Croda glue display identical behaviour on both stationa ry phases as suggested by their principal components. Anomalous behavi our of VM gelatin is suggested by both main factor coefficients and pr incipal component analysis.