IDENTIFICATION AND CHROMOSOMAL ASSIGNMENT OF RAPD MARKERS LINKED WITHA GENE FOR SEED COLOR IN A BRASSICA-CAMPESTRIS-ALBOGLABRA ADDITION LINE

The present study is concerned with a Brassica campestris-alboglabra m onosomic addition line (AA + 1 chromosome from C, 2n = 21) which harbo urs a B. alboglabra (a form of B. oleracea, CC, 2n = 18) chromosome wi th a gene controlling seed colour. Random amplified polymorphic DNA ma rker analysis revealed 19 markers specific to the alien C-genome chrom osome of the addition line. One of these markers was inferred to be cl ose to the seed colour gene. The location of the seed colour gene in a terminal region of the alien chromosome was deduced from what could h ave been homoeologous recombination or chromosomal deletion. Studies o n mitotic prometaphase chromosomes of the addition line indicated that the alien chromosome was apparently chromosome 1 of B. alboglabra. Th e use of the present addition line is discussed in the context of bree ding for yellow-seeded B. alboglabra and B. napus (AACC, 2n = 38).