CLONING AND CHARACTERIZATION OF THE D-TAGATOSE 3-EPIMERASE GENE FROM PSEUDOMONAS-CICHORII ST-24

Authors
ISHIDA Y KAMIYA T ITOH H KIMURA Y IZUMORI K
Citation
Y. Ishida et al., CLONING AND CHARACTERIZATION OF THE D-TAGATOSE 3-EPIMERASE GENE FROM PSEUDOMONAS-CICHORII ST-24, Journal of fermentation and bioengineering, 83(6), 1997, pp. 529-534
Citations number
38
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
83
Issue
6
Year of publication
1997
Pages
529 - 534
Database
ISI
SICI code
0922-338X(1997)83:6<529:CACOTD>2.0.ZU;2-Q
Abstract
The gene encoding D-tagatose 3-epimerase (D-TE) from Pseudomonas cicho rii ST-24 was cloned and sequenced. It was found to consist of 873 bp encoding 290 amino acid residues. The molecular weight of the deduced amino acid sequence of D-TE was determined to be 32.5 kDa. The deduced amino acid sequence showed no extensive homology with sequences of ot her sugar-related epimerases, but homology was observed with several h ypothetical proteins of procaryotes, i.e. Synechocystis sp., Bacillus subtilis, Haemophilus influenzae, and Escherichia coli. The D-TE gene was expressed in E. coil.