POLYUNSATURATED FATTY-ACID PRODUCTION BY RIBES RUBRUM CALLI

Methods of polyunsaturated fatty acid (PUFA) production by plant tissu e culture techniques were investigated. Ribes rubrum calli were induce d from leaves, and the highest rates of callus induction and prolifera tion were observed in B5 modified medium supplemented with 4.0 mg/l 1- naphthalene acetic acid (NAA) and 2.0 mg/l 6-benzyl amino purine (BAP) . PUFA accounted for 53.8% (w/w) of the total higher fatty add content in the calli obtained at a culture temperature of 25 degrees C and 59 .7% in these obtained at a culture temperature of 10 degrees C. It was demonstrated that fats and oils with a high PUFA content were produce d efficiently by the low-temperature treatment.