A NOVEL ACRIDONE DERIVATIVE FOR THE FLUORESCENCE TAGGING AND MASS-SPECTROMETRIC SEQUENCING OF PEPTIDES

Authors
FALLER T HUTTON K OKAFO G GRIBBLE A CAMILLERI P GAMES DE
Citation
T. Faller et al., A NOVEL ACRIDONE DERIVATIVE FOR THE FLUORESCENCE TAGGING AND MASS-SPECTROMETRIC SEQUENCING OF PEPTIDES, Chemical communications, (16), 1997, pp. 1529-1530
Citations number
7
Categorie Soggetti
Chemistry
Journal title
Chemical communications → ACNP
ISSN journal
13597345
Issue
16
Year of publication
1997
Pages
1529 - 1530
Database
ISI
SICI code
1359-7345(1997):16<1529:ANADFT>2.0.ZU;2-K
Abstract
An acridone derivative containing an activated carboxylic group is hig hly reactive towards primary amine groups, and allows selective analys is either using absorbance at a wavelength of 410 nm or fluorescence a t excitation and emission wavelengths of 400 and 418 nm, respectively; MS-MS analysis of peptides tagged with reagent gives fragmentation pa tterns which are usually considerably more intense than background.