In Central Europe seven Ips species are characterized by differences i
n morphology, structure of galleries, host specificity and aggressiven
ess, These species were analysed by allozyme markers and by sequencing
567 bp of the mitochondrial cytochrome oxidase I gene, in order to de
fine their phylogenetic relationships, Orthotomicus erosus and Tomicus
minor were taken as outgroup species. The data revealed high inter-sp
ecific and low intra-specific sequence divergence, Plotting the observ
ed sequence divergence caused by transversions (Tv) and transitions (T
s) and the level of saturation for Ts and Tv of each codon position sh
owed that the third positions were highly saturated by multiple substi
tutions, Maximum parsimony analysis produced two groups: (1) I, typogr
aphus, I. cembrae, I, amitinus, I, duplicatus and I, acuminatus, (2) I
. mannsfeldi, I, sexdentatus and the two outgroups, In all analyses th
e species of the first cluster were put together and I, typographus an
d I, cembrae, and I, mannsfeldi and O, erosus emerged as sister pairs,
The data do not support a common ancestor for the seven European Ips
species, The eight-spined bark beetles (except I, mannsfeldi) and I. a
cuminatus formed a monophyletic group, The close relationship of I, ma
nnsfeldi and O. erosus supports the latter belonging to the genus Ips
as proposed by Wood (1982) and Escherich (1923), However, more genetic
markers and more species of the genera Orthotomicus and Pityokteines
have to be analysed to resolve the phylogenetic positions of I. sexden
tatus and I. mannsfeldi within the tribe Ipini.