DEVELOPMENT OF A NANOSCALE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY METHODOLOGY FOR THE DETECTION AND IDENTIFICATION OF DNA-ADDUCTS

In this work, the coupling of liquid nanochromatography to NanoFlow el ectrospray mass spectrometry was evaluated for the detection of DNA ad ducts, The NanoFlow ES LC/MS system was compared with the capillary an d conventional ES LC/MS system by analyzing an in vitro reaction mixtu re resulting from the interaction of 2'-deoxyguanosine 5'-monophosphat e with bisphenol A diglycidyl ether and by injecting 2'-deoxyadenosine . By using NanoFlow ES LC/MS, the mass sensitivity could be improved b y a factor of 3300. Three different injection methods used in liquid n anochromatography, i.e., split, large-volume, and column-switching inj ections were compared in terms of sensitivity, Furthermore, NanoFlow E S LC/MS was used to detect 2'-deoxynucleotide adducts isolated from an in vitro mixture of calf thymus DNA and bisphenol A diglycidyl ether, Different 2'-deoxynucleotide adducts could be identified by monitorin g typical product ions, diagnostic for the adducts.