S. Farine et al., COMPARATIVE QUANTITATIVE-ANALYSIS OF SUCROSE AND RELATED-COMPOUNDS USING ION-EXCHANGE AND REVERSE-PHASE CHROMATOGRAPHIC METHODS, International journal of biological macromolecules, 21(1-2), 1997, pp. 109-114
Two analytical methods of sugar determination, namely ion exchange chr
omatography on an anionic resin coupled with electrochemical detection
, and reverse phase chromatography on Nucleosil-NH2 resin equipped wit
h a light scattering detector were tested and compared as regards thei
r rapidity, sensitivity and accuracy with sucrose, fructose, glucose,
raffinose, maltose, arabinose, fucose, rhamnose and xylose. Excellent
resolution and highly reproducible results were obtained in both cases
. Greater sensitivity up to the picomolar range was possible however o
nly with ion exchange chromatography. Reverse phase chromatography was
successfully applied to the time course of sucrose hydrolysis under c
hemical (acid) and enzymatic (invertase) conditions. The hydrolysis wa
s monitored by determining sucrose degradation and the corresponding f
ormation of glucose and fructose. (C) 1997 Elsevier Science B.V.