INTEGRATION OF INULIN DETERMINATION IN THE AOAC METHOD FOR MEASUREMENT OF TOTAL DIETARY FIBER

Authors
QUEMENER B THIBAULT JF COUSSEMENT P
Citation
B. Quemener et al., INTEGRATION OF INULIN DETERMINATION IN THE AOAC METHOD FOR MEASUREMENT OF TOTAL DIETARY FIBER, International journal of biological macromolecules, 21(1-2), 1997, pp. 175-178
Citations number
5
Categorie Soggetti
Biology
Journal title
International journal of biological macromolecules → ACNP
ISSN journal
01418130
Volume
21
Issue
1-2
Year of publication
1997
Pages
175 - 178
Database
ISI
SICI code
0141-8130(1997)21:1-2<175:IOIDIT>2.0.ZU;2-A
Abstract
A suitable modification of the standard AOAC method for the measuremen t of dietary fibre is proposed to quantitatively include beta-fructans in the determination of the soluble dietary fibre fraction and as a c onsequence in the related total dietary fibre fraction. The standard A OAC method is modified by including a preheated commercial inulinase, Novozym SP 230(R), to the amyloglucosidase incubation step. It was pre viously outlined that this commercial inulinase contains some pectolyt ic activity. It is now demonstrated that a heat pretreatment of this e nzyme preparation at 60 degrees C for 2 h eliminates this pectolytic a ctivity while keeping sufficient activity to hydrolyse all the inulin from the soluble fibre fraction. (C) 1997 Elsevier Science B.V.