A panel of somatic cell hybrids specific for human chromosome 8 was us
ed to localize 64 expressed sequence-tagged site (ESTS) markers to six
individual regions by PCR amplification. Nine ESTS correspond to 8 kn
own human genes and 6 others show similarities with vertebrate genes,
whereas the remaining 49 ESTS markers correspond to novel genes with n
o database similarities. These gene transcript markers will contribute
to the developing physical and expression maps of chromosome 8, and t
o the search for candidate genes for various human diseases.