PROPAGATION OF HUMAN ASTROVIRUS IN THE PLC PRF/5 HEPATOMA-CELL LINE/

Authors
TAYLOR MB GRABOW WOK CUBITT WD
Citation
Mb. Taylor et al., PROPAGATION OF HUMAN ASTROVIRUS IN THE PLC PRF/5 HEPATOMA-CELL LINE/, Journal of virological methods, 67(1), 1997, pp. 13-18
Citations number
31
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
67
Issue
1
Year of publication
1997
Pages
13 - 18
Database
ISI
SICI code
0166-0934(1997)67:1<13:POHAIT>2.0.ZU;2-Z
Abstract
Astroviruses are associated with gastroenteritis in humans and many di seases in animals. Human astroviruses (HAstVs) cannot be propagated re adily or isolated in conventional cell cultures. The presence of tryps in supports HAstV growth in selected cell cultures such as the continu ous colonic carcinoma cell line (CaCo-2). This study reports on the pr opagation of cell culture adapted reference strains of HAstV, and the direct isolation of HAstV from stool specimens in the human hepatoma c ell line PLC/PRF/5. (C) 1997 Elsevier Science B.V.