RAPID ISOLATION OF DOUBLE-STRANDED RNAS FROM ENTOMOPATHOGENIC SPECIESOF THE FUNGUS PAECILOMYCES USING A COMMERCIAL MINICOLUMN SYSTEM

A method is described for rapid extraction of double-stranded RNAs fro m entomopathogenic fungi. Lyophilised and ground mycelium is incubated with 6 M guanidine thiocyanate, centrifuged, and the cleared lysate a pplied to a QIAGEN silica-based mini-spin column. Following washing wi th 70% isopropanol, bound nucleic acids are eluted under low salt cond itions and treated with DNAse I prior to analysis by non-denaturing ag arose gel electrophoresis. (C) 1997 Elsevier Science B.V.