IMMUNOLOGICAL COMPARISON OF CYTOSOLIC GLUTATHIONE S-TRANSFERASES BETWEEN RAT AND 2 STRAINS OF HOUSEFLIES

Five different antisera, which include three antisera raised against r at liver glutathione S-transferases (GST), one antiserum raised agains t human pi GST, and one antiserum raised against housefly GST1, were u sed to examine their cross-reactivity with different classes of GST su bunits isolated from rat liver and the housefly. Two classes of rat li ver GSTs, alpha and mu, were isolated from rat liver and two classes o f housefly GSTs, GST1 and GST2, were isolated from both CSMA and and C ornell-R strains. Antiserum against GST 3-3 was the most reactive anti serum and reacted not only with the mu class of GSTs but also with the GST1 class from both CSMA and Cornell-R strains. Antiserum against hu man pi GST and antiserum against housefly GST1 had weak immunological reactivity toward the GST1 class from both strains of housefly. Antise rum against GST 4-4 and antiserum against GST 1-1 had no immunological reactivity toward any class of GSTs from housefly. None of the five a ntisera had any immunological cross-reactivity toward subunit 2 of the alpha class of rat GST and the GST2 class of housefly GSTs from both strains.