CHARACTERIZATION AND MAPPING OF SIMPLE SEQUENCE REPEATS (SSRS) IN SORGHUM-BICOLOR

Authors
TARAMINO G TARCHINI R FERRARIO S LEE M PE ME
Citation
G. Taramino et al., CHARACTERIZATION AND MAPPING OF SIMPLE SEQUENCE REPEATS (SSRS) IN SORGHUM-BICOLOR, Theoretical and Applied Genetics, 95(1-2), 1997, pp. 66-72
Citations number
37
Categorie Soggetti
Genetics & Heredity","Plant Sciences
Journal title
Theoretical and Applied Genetics → ACNP
ISSN journal
00405752
Volume
95
Issue
1-2
Year of publication
1997
Pages
66 - 72
Database
ISI
SICI code
0040-5752(1997)95:1-2<66:CAMOSS>2.0.ZU;2-Y
Abstract
A total of 13 SSR loci were characterized in Sorghum bicolor. Ten of t hese loci were isolated by screening sorghum genomic AG-enriched libra ries with labelled poly(AC)/poly(CT), the other three were derived fro m database searches. In order to explore the degree of polymorphism de tectable in this species by this type of molecular marker: the SSR mar kers were tested on nine inbred lines of S. bicolor of different geogr aphic origin. PCR analysis on acrylamide gels revealed a high degree o f polymorphism (delta(T) = 0.80), One locus, in particular, allowed th e identification of all of the nine inbred lines used in our study. Se ven of these SSR markers were mapped, using an existing sorghum RFLP m ap.