DIFFERENTIAL EXPRESSION OF 2 GENES FOR SUCROSE-PHOSPHATE SYNTHASE IN SUGARCANE - MOLECULAR-CLONING OF THE CDNAS AND COMPARATIVE-ANALYSIS OFGENE-EXPRESSION

Two cDNA clones, pSoSPS1 and pSoSPS2, encoding sucrose-phosphate synth ase (SPS) of sugarcane were isolated from a leaf cDNA library. Norther n analysis revealed the transcript of SoSPS1 to be predominant in leav es, but that of SoSPS2 to be distributed not only in leaves but also i n roots at a similar level. The transcript of SoSPS1 was markedly accu mulated during greening of etiolated leaves, whereas that of SoSPS2 wa s constitutively expressed. These findings indicate that SPS in sugarc ane is encoded by multiple genes, which show organ-specificity and are differentially regulated in response to light.