Dcs. Huang et al., THE ANTI-APOPTOSIS FUNCTION OF BCL-2 CAN BE GENETICALLY SEPARATED FROM ITS INHIBITORY EFFECT ON CELL-CYCLE ENTRY, EMBO journal, 16(15), 1997, pp. 4628-4638
The Bcl-2 family of proteins regulate apoptosis, some antagonizing cel
l death and others facilitating it, It has recently been demonstrated
that Bcl-2 not only inhibits apoptosis but also restrains cell cycle e
ntry, We show here that these two functions can be genetically dissoci
ated, Mutation of a tyrosine residue within the conserved N-terminal B
H4 region had no effect on the ability of Bcl-2 or its closest homolog
s to enhance cell survival and did not prevent heterodimerization with
death-enhancing family members Bar, Bah, Bad and Bik. Neither did thi
s mutation override the growth-inhibitory effect of p53, However, on s
timulation with cytokine or serum, starved quiescent cells expressing
the mutant proteins re-entered the cell cycle much faster than those e
xpressing comparable levels of wildtype proteins. When wild-type and Y
28 mutant Bcl-2 were co-expressed, the mutant was dominant, Although R
-Ras p23 has been reported to bind to Bcl-2, no interaction was detect
able in transfected cells and R-Ras p23 did not interfere with the abi
lity of Bcl-2 to inhibit apoptosis or cell cycle entry, These observat
ions provide evidence that the anti-apoptotic function of Bcl-2 is mec
hanistically distinct from its inhibitory influence on cell cycle entr
y.