STRUCTURE ELUCIDATION OF GLYKENIN GLYCOSIDIC ANTIBIOTICS FROM BASIDIOMYCETES SP .5. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF COMPONENTS OF GLYKENIN

The glycosidic antibiotics of the glykenin (GK) family produced by Bas idiomycetes sp. were separated into nine components (GK-I-VII and DG) by normal-phase chromatography. It was found that these components dif fer in the number and location of the acetyl groups in the sugar moiet y. Each component (GK-I-VII and DG) was further separated into three i somers (A, B and C), which possess different aglycones, by reversed-ph ase chromatography on an ODS column with methanol-acetonitrile as elue nt. The best composition of the eluent was found to be methanol-aceton itrile-1% trifluoroacetic acid (4:3.5:2.5). The profile analysis of GK -III-VII and DG was also carried out using a modified mobile phase. Th e combination of normal- and reversed-phase chromatography separated a ll components of the GK mixture except GK-I and II. The relationship b etween structure and separation behaviour of GK is discussed.