SOOTY FOOT, A NOVEL MOUSE MUTATION THAT AFFECTS THE PIGMENTATION OF EXPOSED SKIN, BUT NOT HAIR, MAPS TO CHROMOSOME-2

We have characterized a novel recessive mouse mutation, named sooty fo ot, that increases the pigmentation of the exposed skin on the foot pa ds, the genital region, around the snout and muzzle, the ears, and the tail. By contrast, the pigmentation of the hair is unaffected. We hav e localized the mutation to Chromosome 2 by polymerase chain reaction (PCR) amplification of simple sequence repeats from pooled DNA from ba ckcross progeny. In an extended backcross we have generated a detailed map of the region around sooty foot.