CHARACTERIZATION OF THE MOUSE BETA-PRIME ADAPTIN GENE - CDNA SEQUENCE, GENOMIC STRUCTURE, AND CHROMOSOMAL LOCALIZATION

Adaptins are important subunits of heterotetrameric complexes called a daptors, which participate in the clathrin-coated, vesicle-mediated en docytosis and intracellular receptor transport. The gene family of ada ptins is divided into three classes, alpha, beta, and gamma, with furt her subdivision into beta and beta-prime components. Two beta-prime ad aptins, the rat AP105a and the human BAM22, have previously been chara cterized. The BAM22 gene is located on human Chromosome (Chr) 22q12 an d can be considered a candidate meningioma tumor suppressor gene. We r eport here the characterization of the mouse ortholog of the BAM22 gen e, and we suggest the name adtb1 for the mouse gene. Like the BAM22 ge ne, the adtb1 transcript is highly and ubiquitously expressed. We prov ide 3885-bp cDNA sequence, which entirely covers the open reading fram e of the adtb1, capable of encoding a protein of 943 amino acids. The adtb1 protein is highly conserved (>96% identity) when compared with A P105a and BAM22 proteins. We also report the genomic organization of a dtb1, which is similar to the BAM22 gene. The adtb1 gene has been assi gned to mouse Chr 11, band 11A2, which confirms the synteny between hu man Chr 22q12 and mouse Chr 11.