ENANTIOMERIC PROPERTIES OF HUMAN ALBUMIN IMMOBILIZED ON POROUS SILICASUPPORTS COATED WITH POLYMETHACRYLOYL CHLORIDE

Human serum albumin (HSA) was bound to porous silica, using a reactive polymer derived from polymethacryloyl chloride. Two different procedu res were used for coating silica with the polymer. In the first method , the polymer was deposited onto amino-silica by reaction between its reactive functions and NH, groups on silica. In the second method, the monomer was first linked to the amino-silica and copolymerization wit h the excess of monomer initiated thereafter. The enantiomeric propert ies of the resulting supports after the coupling of HSA were compared using different mobile phases. The higher amount of HSA bound using th e later method, resulted in higher retention of the enantiomers and be tter enantioselectivity. (C) 1997 Elsevier Science B.V.