ENANTIOSELECTIVITY PROPERTIES OF HUMAN SERUM-ALBUMIN IMMOBILIZED ON ANION-EXCHANGERS BASED ON POLYVINYLIMIDAZOLE-COATED SILICA - EFFECT OF PROTEIN LOADING ON SEPARATION PROPERTIES

Chiral chromatographic supports were obtained by continuously applying solutions contained HSA to ion-exchange columns. The columns were pac ked with silica modified with polyvinylimidazole and a copolymer polyv inylpyrrolidone-polyvinylimidazole (75:25) respectively, quaternized a nd crosslinked. Small changes in the concentration of NaCl during immo bilization of HSA lead to variations in the amount of HSA bound to the supports. These variations have consequences in terms of chromatograp hic retention (k'), selectivity (alpha) and resolution (R-s) of enanti omers. The effects of varying the pH and organic modifier of the mobil e phase on the chromatographic properties were also examined. (C) 1997 Elsevier Science B.V.