PEPTIDE BANK GENERATED BY LARGE-SCALE PREPARATION OF CIRCULATING HUMAN PEPTIDES

Human hemofiltrate (HF) is a source for the purification of circulatin g regulatory peptides. HF is obtained in large quantities during treat ment of patients suffering from chronic renal failure. We have develop ed a large-scale method for separating peptides from amounts up to 10 000 1 HF into 300 fractions in a standardized two-step procedure, empl oying cation-exchange separation, followed by reversed-phase chromatog raphy. These fractions represent a peptide bank containing bioactive, desalted and lyophilized peptides of blood. Screening for and isolatio n of regulatory human peptides is simplified by using this peptide ban k. (C) 1997 Elsevier Science B.V.